suggested use of this reagent is 0.5 µg per 106 cells in 100 µl
It is recommended that the reagent be titrated for optimal performance for
recognizes a 50KDa lymphocyte surface antigen which is expressed on all
peripheral blood T lymphocytes,the majority of lymphocytes and malignant cells of T cell origin, including T ALL
cells. Normal B lymphocytes, monocytes or
granulocytes do not express surface CD2 antigen, neither do common ALL cells.
CD2 antigen has been characterised as the receptor
for sheep erythrocytes. This CD2 monoclonal antibody inhibits E rosette
formation. CD2 antigen also functions as the receptor for the CD58 antigen(LFA-3).