Use 10µl for 106 cells
CD4, also known as T4, is a 55 kD
single chain transmembrane glycoprotein and belongs to immunoglobulin
superfamily. CD4 is found on most thymocytes, a subset of T cells and at
low level on monocytes/macrophages.
CD4 is a co-receptor involved in immune response (co-receptor activity
in binding to MHC class II molecules) and HIV infection (CD4 is primary
receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell
activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection
and signal transduction.