dilutions. WB: 1/1000, ELISA:1/4000
*Each investigator is highly recommended to titrate the reagent to
identify the optimal concentration for each application.
Epitope tags are commonly used for
labeling, detection and purification of recombinant proteins. Due to
their small size, they are unlikely to affect the biochemical properties
of the tagged fusion proteins.
Glutathione S transferases (GSTs) are a family of enzymes that play an
important role in detoxification by catalyzing the conjugation of many
hydrophobic and electrophilic compounds with reduced glutathione. The
amino acid sequence GST is highly conserved in most organisms, including
mammals. GST is a widely used fusion partner, since it provides both an
easily detectable Tag and a simple purification process of interest.
GeneMay’s monoclonal antibody, clone GMW.15, is developed using
recombinant GST expressed in E. Coli. as antigen. It recognizes GST
tagged fusion protein in different expression system.