FACS: The suggested use of this reagent is 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
CB19 reacts with CD19 (B4), a 90 kDa molecule, which is expressed on approximately 5-25% of human peripheral blood lymphocytes. CD19 antigen is present on human B lymphocytes at most stages of maturation, from the earliest Ig gene rearrangement in pro-B cells to mature cell, as well as malignant B cells, but is lost on maturation to plasma cells. CD19 antibody does not react with T lymphocytes, monocytes and granulocytes. CD19 is a critical signal transduction molecule that regulates B lymphocyte development, activation and differentiation.This clone is cross reactive with non-human primate. * CD19 is a key phenotyping marker of non-T cell leukemia.
