dilutions. WB: 1/1000, ELISA:1/4000
*Each investigator is highly recommended to titrate the reagent to
identify the optimal concentration for each application.
Epitope tags are commonly used for
labeling, detection and purification of recombinant proteins. Due to
their small size, they are unlikely to affect the biochemical properties
of the tagged fusion proteins.
The His tag on the expressed recombinant proteins allows for efficient
coupling to Ni++ affinity resins and purification by a single step
chromatography. GeneMay’s polyclonal antibody is developed by using
synthetic peptide C-HHHHHH conjugated to KLH as antigen. It recognizes
His-tagged fusion protein in different expression system.