dilutions. WB: 1/1000, ELISA:1/4000
*Each investigator is highly recommended to titrate the reagent to
identify the optimal concentration for each application.
Epitope tags are commonly used for
labeling, detection and purification of recombinant proteins. Due to
their small size, they are unlikely to affect the biochemical properties
of the tagged fusion proteins.
The V5 epitope tag is derived from a small epitope present on the P/V
proteins of the paramyxovirus of simian virus 5 (SV5). GeneMay’s
monoclonal antibody, clone GM14H, is developed using synthetic peptide
C-GKPIPNPLLGLDST conjugate to KLH as antigen. It recognizes V5-tagged
fusion protein in different expression system.